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Tips for effective use of BLAST and other NCBI tools

The National Center for Biotechnology Information (NCBI) provides one of the most extensive sets of web-based tools for biological research. The tools are indispensable when planning genomics experiments, including for qPCR, NGS, and CRISPR. In this presentation, Dr Matt McNeill will take a practical look at getting started with the wealth of NCBI tools, and share some relevant tips to help you sift through the tools and options that we regularly use. In particular, he will focus on commonly adjusted parameters that will allow you to more effectively use the powerful Basic Local Alignment Algorithm Tool (BLAST) to identify off-target hybridization/annealing events. Dr McNeill will cover practical examples using NCBI tools to design assays and, following the presentation, we will answer questions from attendees.

DATE:
Tuesday, August 23, 2016

TIME:
Session 1: 9:00 am CDT (UTC-5 hours)
Session 2: 1:00 pm CDT (UTC-5 hours)

unnamedPresenter: Matthew McNeill, PhD

Staff Scientist, Bioinformatics

Integrated DNA Technologies

Dr Matthew McNeill is a staff scientist in the Bioinformatics group at IDT. Dr McNeill earned his PhD in Neuroscience from the University of Iowa, where he studied the role of the cation channel, TRPM7, in zebrafish brain and skin development. As a postdoctoral researcher at the University of Illinois, he studied social behavior in honey bees using RNAseq and mRNA in situ hybridization. At IDT, Dr McNeill has primarily focused on development of our NGS product line.

 

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